DAPI
powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence
کد معادل: D9542 sigma
Application
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Suitable for
DNA staining in agarose gels
analysis of changes in DNA during apoptosis
detection of mycoplasma
photofootprinting of DNA
immunofluorescent staining of cells
DAPI has been used:
in rapid monitoring of microbial contamination
in chromosomal banding technique
in detection of apoptotic cells
in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
to stain mature pollen grains(0.5 mg/ml)
About This Item
Formula : C16H15N5 · 2HCl
CAS Number: 28718-90-3
Molecular Weight: 350.25
Assay: ≥98% (HPLC)
form: powder
technique(s): transfection: suitable
solubility: H2O: 20 mg/mL, PBS: insoluble
storage temp: 2-8°C
