Poly-L-Lysine Hydrobromide
synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra
کد معادل: P9155 Sigma
Description
Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.
Application
Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates. It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.
About This Item
CAS Number: 25988-63-0
form: lyophilized powder
mol wt: 30,000-70,000
solubility: water
storage temp:-20°C
technique(s): cell culture | mammalian: suitable
