Sodium dodecyl sulfate (SDS)
BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
کد معادل: L3771 Sigma
Application
in chromatin immunoprecipitation
in SDS-polyacrylamide gel electrophoresis (SDS-PAGE)
as a component of radioimmunoprecipitation assay buffer for immunoblotting
Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.
About This Item
Formula: CH3(CH2)11OSO3Na
CAS Number: 151-21-3
Molecular Weight: 288.38
grade: for molecular biology
description: anionic
form: powder
technique(s):electrophoresis: suitable
solubility: water
storage temp: room temp