Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase substrate has been used as a substrate in tartrate-resistant acid phosphatase (TRAP) assay and alkaline phosphatase (ALP).
About This Item
Formula: O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number: 333338-18-4
Molecular Weight: 371.14
form: powder
solubility: water: 100 mg/mL, clear to slightly hazy
storage temp: -20°C
